Basic Information

Strain Name
C57BL/6N-Epotm1(EPO)Bcgen/Bcgen
Common Name
B-hEPO mice
Background
C57BL/6N
Catalog Number
112458
NCBI Gene ID
Related Genes
EPO: EP; DBAL; ECYT5; MVCD2

Targeting strategy

Gene targeting strategy for B-hEPO mice.
The exons 1-5 of mouse Epo gene that encodes the full-length protein was replaced by human EPO exons 1-5 in B-hEPO mice.

Protein expression analysis

Strain specific EPO expression analysis in homozygous B-hEPO mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEPO mice (H/H) and analyzed by ELISA with species-specific EPO ELISA kit. Mouse EPO was detectable in wild-type mice (A). Human EPO was exclusively detectable in homozygous B-hEPO mice but not in wild-type mice (B).

Summary

Protein expression analysis:
Human EPO was detectable on serum of homozygous B-hEPO mice, and mouse EPO was detectable only in wild-type C57BL/6 mice.

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