Basic Information
Strain Name
C57BL/6N-Epotm1(EPO)/Bcgen
Common Name
B-hEPO mice
Background
C57BL/6N
Catalog Number
112458
NCBI Gene ID
Related Genes
EPO: EP; DBAL; ECYT5; MVCD2
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Targeting strategy
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Gene targeting strategy for B-hEPO mice.
The exons 1-5 of mouse Epo gene that encodes the full-length protein was replaced by human EPO exons 1-5 in B-hEPO mice. -
Protein expression analysis
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Strain specific EPO expression analysis in homozygous B-hEPO mice by ELISA. Serum were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hEPO mice (H/H) and analyzed by ELISA with species-specific EPO ELISA kit. Mouse EPO was detectable in wild-type mice (A). Human EPO was exclusively detectable in homozygous B-hEPO mice but not in wild-type mice (B).
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Summary
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Protein expression analysis:
Human EPO was detectable on serum of homozygous B-hEPO mice, and mouse EPO was detectable only in wild-type C57BL/6 mice.
