Basic Information
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Targeting strategy
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Gene targeting strategy for B-hF3 mice.
The exons 1-6 of mouse F3 gene that encode the extracellular domain were replaced by human F3 exons 1-6 in B-hF3 mice.
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Protein expression analysis in lung
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Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry.
Endotheliocytes from lung were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.
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Protein expression analysis in brain
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Strain specific F3 expression analysis in homozygous B-hF3 mice by flow cytometry.
Endotheliocytes from brain were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hF3 mice (H/H), and analyzed by flow cytometry with species-specific anti-F3 antibody. Mouse F3 was detectable in wild-type mice. Human F3 was exclusively detectable in homozygous B-hF3 mice but not in wild-type mice.
