Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGDF8 mice.
Exons 1-3 of the mouse Gdf8 gene, which encode the full-length protein, were replaced by human GDF8 exon 1-3 in B-hGDF8 mice.
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Protein expression analysis
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GDF8 expression analysis in homozygous B-hGDF8 mice by ELISA. Plasma was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), and analyzed by ELISA. Human and mouse GDF8 proteins were both detectable in wild-type C57BL/6 and B-hGDF8 mice. The ELISA kit cross-recognizes human and mouse GDF8.
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mRNA expression analysis
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Strain specific analysis of GDF8 mRNA expression in wild-type C57BL/6 mice and B-hGDF8 mice by RT-PCR. Skeletal muscle RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGDF8 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GDF8 primers. Mouse Gdf8 mRNA was detectable only in wild-type C57BL/6 mice. Human GDF8 mRNA was detectable in homozygous B-hGDF8 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Gdf8 mRNA was detectable in the skeletal muscle of wild-type C57BL/6 mice. Human GDF8 mRNA was detectable in B-hGDF8 mice.
Protein expression analysis:
Human and mouse GDF8 proteins were detectable in wild-type C57BL/6 and B-hGDF8 mice. The ELISA kit cross-recognizes human and mouse GDF8.
