B-hGEP mice

Basic Information

Targeting strategy

Gene targeting strategy for B-hGEP mice.
The exons 2-13 of mouse Gep gene that encode the full-length protein were replaced by human GEP exons 2-13 in B-hGEP mice.

Protein expression analysis

Strain specific GEP expression analysis in homozygous B-hGE mice by ELISA. Serum were collected from wild-type mice C57BL/6 mice (+/+) and homozygous B-hGEP mice (H/H), and analyzed by ELISA with species-specific Progranulin ELISA kit. Mouse Progranulin was detectable in wild-type mice (A). Human Progranulin was exclusively detectable in homozygous B-hGEP mice but not in wild-type mice (B).

Summary

Protein expression analysis:
Mouse Progranulin was detectable in wild-type mice. Human Progranulin was exclusively detectable in homozygous B-hGEP mice but not in wild-type mice.