Basic Information

Strain name
C57BL/6-Igfbp2tm1(IGFBP2)/Bcgen
Common name
B-hIGFBP2 mice
Catalog number
110987
Background
C57BL/6
Aliases
IGFBP2 (IBP2,IGF-BP53)

Description

The protein encoded by this gene is one of several similar proteins that bind insulin-like growth factors I and II (Igf-I and Igf-II). The encoded protein can be secreted into the bloodstream, where it binds Igf-I and Igf-II with high affinity, or it can remain intracellular, interacting with many different ligands. Two transcript variants, one encoding a secreted isoform and the other encoding a nonsecreted isoform, have been found for this gene.

Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Homozygous mutation of this gene results in reduced spleen, heart and kidney size and increased liver weight. Homozygotes for another allele exhibit a normal phenotype. This is a potential target in the field of cancer treatment.

Targeting strategy

The exons 1-4 of mouse Igfbp2 gene that encodes the full-length protein was replaced by human IGFBP2 exons 1-4 in B-hIGFBP2 mice.

Protein expression analysis

Strain specific IGFBP2 expression analysis in heterozygous B-h IGFBP2 mice by Western Blot. Liver tissue was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hIGFBP2 mice (H/+) , and analyzed by western blot with species-specific IGFBP2 antibody. Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice. Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.

References

  1. Massoner P , Ladurner-Rennau M , Eder I E , et al. Insulin-like growth factors and insulin control a multifunctional signalling network of significant importance in cancer[J]. British Journal of Cancer, 2010, 103(10):1479-1484.