Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hIGFBP2 mice. The exons 1-4 of mouse Igfbp2 gene that encodes the full-length protein was replaced by human IGFBP2 exons 1-4 in B-hIGFBP2 mice.
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Protein Expression Analysis
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Strain specific IGFBP2 expression analysis in heterozygous B-h IGFBP2 mice by Western Blot. Liver tissue was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hIGFBP2 mice (H/+) , and analyzed by western blot with species-specific IGFBP2 antibody. Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice. Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.
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Summary
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Protein expression analysis:
Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice.
Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.
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References
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- Massoner P , Ladurner-Rennau M , Eder I E , et al. Insulin-like growth factors and insulin control a multifunctional signalling network of significant importance in cancer[J]. British Journal of Cancer, 2010, 103(10):1479-1484.
