Basic Information

Strain name
C57BL/6-Igfbp2tm1(IGFBP2)/Bcgen
Common name
B-hIGFBP2 mice
Catalog number
110987
Background
C57BL/6J
NCBI Gene ID
Aliases
IGFBP2 (IBP2,IGF-BP53)

Targeting strategy

The exons 1-4 of mouse Igfbp2 gene that encodes the full-length protein was replaced by human IGFBP2 exons 1-4 in B-hIGFBP2 mice.

Protein expression analysis

Strain specific IGFBP2 expression analysis in heterozygous B-h IGFBP2 mice by Western Blot. Liver tissue was collected from wild-type C57BL/6 mice (+/+) and heterozygous B-hIGFBP2 mice (H/+) , and analyzed by western blot with species-specific IGFBP2 antibody. Mouse IGFBP2 was detectable in wild type mice and heterozygous B-hIGFBP2 mice, as the antibody is crossly reactive with IGFBP2 in human and mice. Human IGFBP2 was exclusively detectable in heterozygous B-hIGFBP2 mice but not wild-type mice.

References

  1. Massoner P , Ladurner-Rennau M , Eder I E , et al. Insulin-like growth factors and insulin control a multifunctional signalling network of significant importance in cancer[J]. British Journal of Cancer, 2010, 103(10):1479-1484.