Basic Information
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Targeting strategy
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Gene targeting strategy for B-hIL12RB1/hIL12RB2 mice ad.
uThe extracellular region sequences of mouse Il12rb1 were replaced by human IL12RB1 counterpart gene sequences in B-hIL12RB1/hIL12RB2 mice ad.
uChimeric CDS, composed of human IL12RB2 extracellular region plus mouse Il12rb2 transmembrane and cytoplasmic region, was inserted into the mouse Il12rb2 gene exon2 in B-hIL12RB1/hIL12RB2 mice ad.
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Function analysis
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IL12 induced the IFN-γ production in CD4+ T cells sorted from splenocytes. CD4+ T cells were sorted from the splenocytes of wild-type C57BL/6 mice, homozygous B-hIL12RB1/hIL12RB2 mice plus, homozygous B-hIL12RB1/hIL12RB2 mice ad and homozygous B-hIL12A/hIL12B/hIL12RB1/hIL12RB2 mice. The production of IFN-γ in supernatants were assessed after incubation with rm/hIL-12 in combination with bead-associated 0.4 μg/mL anti-mCD3e and 0.8 μg/mL anti-mCD28 antibodies for 48 hours. Mouse IFN-γ were increased after responsiveness to mIL-12 in humanized mice and wild-type mice. While, only hIL-12 induced mouse IFN-γ increase in humanized mice. Mice information: 1) humanized mice, male, 3 mice/group, 14-week-old. 2) wild-type C57BL/6 mice, 2 female and 1 male mice, 14-week-old.
