Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hIL27RA mice. The extracellular gene sequences of mouse Il27ra gene were replaced with human IL27RA counterpart gene in B-hIL27RA mice.
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mRNA expression analysis
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Strain specific analysis of IL27RA gene expression in wild-type mice and B-hIL27RA mice by RT-PCR. Mouse IL27RA mRNA was detectable in the spleen of wild-type mice (+/+). Human IL27RA mRNA was detectable only in homozygous B-hIL27RA but not in wild-type mice.
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Protein expression analysis
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Strain specific IL27RA expression analysis in homozygous B-hIL27RA mice by flow cytometry. CD4+ T cells were collected from wild type mice (+/+) and homozygous B-hIL27RA mice (H/H) stimulated with anti-mCD3ε and anti-mCD28 in vitro for 72h, and analyzed by flow cytometry with species-specific anti-IL27RA antibody. Mouse IL27RA was exclusively detectable in wild-type mice and human IL27RA was detectable in homozygous B-hIL27RA mice.
