Gene targeting strategy for B-hIL27RA mice. The extracellular gene sequences of mouse Il27ra gene were replaced with human IL27RA counterpart gene in B-hIL27RA mice.
Protein expression analysis
Strain specific IL27RA expression analysis in homozygous B-hIL27RA mice by flow cytometry. CD4+ T cells were collected from wild type mice (+/+) and homozygous B-hIL27RA mice (H/H) stimulated with anti-mCD3ε and anti-mCD28 in vitro for 72h, and analyzed by flow cytometry with species-specific anti-IL27RA antibody. Mouse IL27RA was exclusively detectable in wild-type mice and human IL27RA was detectable in homozygous B-hIL27RA mice.