IL4 (interleukin 4) encoded by this gene is a pleiotropic cytokine produced by activated T cells. IL4 is capable of binding to its receptor IL4R, while IL13 is also capable of binding to IL4R, which may result in a functional overlap of IL4 and IL13. IL-4 has immunomodulatory effects on B cells, T cells, mast cells, macrophages, and the like. It can promote the proliferation of antigen or mitogen-activated B cells; promote T cell activity, single IL-4 can not stimulate macrophage proliferation, but can enhance macrophage function.
Gene targeting strategy for B-hIL4 mice. The exons 1-4 of mouse IL4 gene that encode the whole sequence were replaced by human IL4 exons 1-4 in B-hIL4 mice.
mRNA expression analysis
Strain specific analysis of IL4 gene expression in WT and hIL4 mice by RT-PCR. Mouse IL4 mRNA was detectable in splenocytes of wild-type (+/+) but not in homozygous (H/H) mice. Human IL4 mRNA was detectable only in homozygous (H/H) but not in wild-type (+/+) mice.
Protein expression analysis
Strain specific IL4 expression analysis in homozygous B-hIL4 mice by ELISA.
Serum were collected from WT and homozygous B-hIL4 (H/H) mice stimulated with LPS in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse Il6 was detectable in WT mice. Human IL4 was exclusively detectable in homozygous B-hIL4 but not WT mice.