Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hIL9R mice. The exons 2-7 of mouse Il9r gene that encode the extracellular coding sequence were replaced by human IL9R exons 2-7 in B-hIL9R mice.
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mRNA Expression Analysis
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Strain specific analysis of IL9R gene expression in B-hIL9R mice by RT-PCR. Mouse Il9 was detectable in splenocytes of wild-type mice (+/+). Human IL9R was detectable only in homozygous B-hIL9R mice but not in wild-type mice.
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Protein expression analysis
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Strain specific IL9R expression analysis in homozygous B-hIL9R mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL9R mice (H/H) stimulated with anti-CD40 in vitro (10 μg/mL), and analyzed by flow cytometry with species-specific anti-IL9R antibody. Mouse IL9R was detectable in wild-type mice. Human IL9R was exclusively detectable in homozygous B-hIL9R but not in wild-type mice.
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Summary
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mRNA expression in B-hIL9R mice:
Human IL9R was detectable only in splenocytes of homozygous B-hIL9R mice but not in wild-type mice.
Protein expression in B-hIL9R mice mice:
Human IL9R was exclusively detectable in splenocytes of homozygous B-hIL9R mice but not in wild-type mice.
