Basic Information

Strain Name
C57BL/6-Il9rtm1(IL9R)/Bcgen
Common Name
B-hIL9R mice
Catalog number
110761
Background
C57BL/6
Aliases
CD129, IL-9R

Description

Interleukin 9 receptor (IL9R) also known as CD129 (Cluster of Differentiation 129) is a type I cytokine receptor. The protein encoded by this gene is a cytokine receptor that specifically mediates the biological effects of interleukin 9 (IL9). The functional IL9 receptor complex requires this protein as well as the interleukin 2 receptor, gamma (IL2RG), a common gamma subunit shared by the receptors of many different cytokines. The ligand binding of this receptor leads to the activation of various JAK kinases and STAT proteins, which connect to different biologic responses. This gene is located at the pseudoautosomal regions of X and Y chromosomes. Genetic studies suggested an association of this gene with the development of asthma.

Targeting strategy

The exons 2-7 of mouse Il9r gene that encode the extracellular coding sequence were replaced by human IL9R exons 2-7 in B-hIL9R mice.

mRNA expression analysis

Strain specific analysis of IL9R gene expression in B-hIL9R mice by RT-PCR. Mouse Il9 was detectable in splenocytes of wild-type mice (+/+). Human IL9R was detectable only in homozygous B-hIL9R mice but not in wild-type mice.

Protein expression analysis

Strain specific IL9R expression analysis in homozygous B-hIL9R mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and homozygous B-hIL9R mice (H/H) stimulated with anti-CD40 in vitro (10 μg/mL), and analyzed by flow cytometry with species-specific anti-IL9R antibody. Mouse IL9R was detectable in wild-type mice. Human IL9R was exclusively detectable in homozygous B-hIL9R but not in wild-type mice.