Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hLAIR1 mice. The exons 3-4 of mouse Lair1 gene that encode the extracellular domain were replaced by human LAIR1 exons 3-6 in B-hLAIR1 mice.
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Protein expression analysis
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Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Splenic T cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Splenic NK cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Splenic monocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Splenic macrophages were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Blood T cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Blood NK cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Blood monocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected in B-hLAIR1 mice.
Species-specific LAIR1 protein expression analysis in wild-type and humanized B-hLAIR1 mice. Blood macrophages were isolated from wild-type C57BL/6 (+/+) and homozygous B-hLAIR1 (H/H) mice and analyzed by flow cytometry using species-specific anti-LAIR1 antibodies. Murine LAIR1 protein was detected in wild-type mice, while human LAIR1 protein was detected B-hLAIR1 mice.
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BLM induced skin fibrosis
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Mouse body weight changes over the study period. Body weight and percentage changes of mice during the experiment in B-hLAIR1 mice. N=6 mice per group.
- Dermis thickness of skin. Representive image of HE staining and dermis thickness change in B-hLAIR1 mice. N=6 mice per group.
- Positive area of Masson staining. Representive image of Masson staining and percentage change of Masson positive tissue area in B-hLAIR1 mice. N=6 mice per group.
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Zymosan induced sub-acute peritonitis
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Body weight changes in Zymosan induced peritonitis models. The body weight percentage relative to the day of grouping (Day 0) are illustrated. Two way ANOVA with multiple comparison test, all groups compared with each other. Values are expressed as mean ± SEM. Significances are separately listed in the table (only meaningful comparisons are listed, i.e. significant comparison between groups with single independent variable in design). Body weights for animals in G1-G8 were not illustrated as modeling and sampling occurred on the same day.
Increased CD45+ cells in peritoneal lavage observed in Zymosan induced sub-acute peritonitis in C57BL/6 and B-hLAIR1 mice. Peritoneal lavage were collected 3 days post zymosan injection for flow cytometry analysis for G9-G16. The total count of CD45+ cells were illustrated. Significantly increased CD45+ cells can be observed in both C57BL/6 mice and B-hLAIR1 mice administered 10 mg zymosan-D. For B-hLAIR1 mice, 1 mg zymosan, 10 mg zymosan also significantly increased CD45+ cells, though this is not observed in the C57BL/6 mice. One-way ANOVA followed by multiple comparison, all groups compared with each other. Values are expressed as mean ± SEM.
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Zymosan induced acute peritonitis
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Increased CD45+ cells in peritoneal lavage observed in Zymosan induced acute peritonitis in C57BL/6 and B-hLAIR1 mice. Peritoneal lavage were collected 4 hours post zymosan injection for flow cytometry analysis for G1-G8. The total count of CD45+ cells were illustrated. Significantly increased CD45+ cells can be observed in both C57BL/6 mice and B-hLAIR1 mice administered 1 mg zymosan. For C57BL/6 mice, 0.1 mg zymosan also significantly increased CD45+ cells, though this is not observed in the B-hLAIR1 mice. One-way ANOVA followed by multiple comparison, all groups compared with each other. Values are expressed as mean ± SEM.
