Basic Information
Strain Name
C57BL/6-Lypd3tm1(LYPD3)Bcgen/Bcgen
Stock Number
111661
Common Name
B-hLYPD3 mice
Aliases
LYPD3: C4.4A
Background
C57BL/6
NCBI Gene ID
-
mRNA expression analysis
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Strain specific analysis of LYPD3 mRNA expression in wild-type C57BL/6 mice and B-hLYPD3 mice by RT-PCR. Stomach RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hLYPD3 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LYPD3 primers. Mouse Lypd3 mRNA was detectable only in wild-type mice. Human LYPD3 mRNA was detectable only in homozygous B-hLYPD3 mice.
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Protein expression analysis
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Western blot analysis of LYPD3 protein expression in homozygous B-hLYPD3 mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hLYPD3 mice (H/H), and then analyzed by western blot with anti-LYPD3 antibody. 40 μg total proteins were loaded for western blotting analysis.
