Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPCSK9 mice plus. The genome of mouse Pcsk9 gene including 5’UTR and 3’UTR was replaced by human PCSK9 genome including 5’UTR and 3’UTR in B-hPCSK9 mice plus.
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mRNA expression analysis in humanized B-hPCSK9 mice plus
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Strain specific analysis of PCSK9 gene expression in wild-type mice and B-hPCSK9 mice plus by RT-PCR. Liver was collected from wild-type mice and heterozygous B-hPCSK9 mice plus. Human PCSK9 mRNA was detectable only in heterozygous B-hPCSK9 mice plus (H/+) but not in wild-type mice (+/+).
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Protein expression analysis in serum
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Strain specific PCSK9 expression analysis in heterozygous humanized B-hPCSK9 mice plus by ELISA. Serum was collected from heterozygous B-hPCSK9 mice plus (H/+) (n=1). Protein expression level of PCSK9 was analyzed by ELISA. Mouse PCSK9 and human PCSK9 were detectable in heterozygous B-hPCSK9 mice plus (H/+).
