Basic Information
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mRNA expression analysis
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Strain specific analysis of RNASE4 mRNA expression in wild-type C57BL/6 mice and B-hRNASE4 mice by RT-PCR. Lung RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hRNASE4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human RNASE4 primers. Mouse Rnase4 mRNA was detectable only in wild-type C57BL/6 mice. Human RNASE4 mRNA was detectable in homozygous B-hRNASE4 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of RNASE4 protein expression in homozygous B-hRNASE4 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hRNASE4 (H/H) mice, and then analyzed by western blot with anti-RNASE4 antibody. 40 μg total protein was loaded for western blotting analysis. RNASE4 was detected in bladder.
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Summary
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mRNA expression analysis:
Mouse Rnase4 mRNA was detectable in the brain of wild-type C57BL/6 mice. Human RNASE4 mRNA was detectable in B-hRNASE4 mice.
Protein expression analysis:
RNASE4 was detected in bladder of wild-type C57BL/6 mice and B-hRNASE4 mice.
