Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hSPP1 mice. The full coding region of mouse Spp1 gene was replaced by the full coding sequence of human SPP1 gene in B-hSPP1 mice.
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mRNA expression analysis
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Strain specific analysis of SPP1 gene expression in wild-type and B-hSPP1 mice by RT-PCR. Mouse Spp1 gene was detectable in splenocytes of wild-type mice (+/+). Human SPP1 gene was detectable only in homozygous B-hSPP1 mice (H/H).
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Protein expression analysis
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Strain specific SPP1 expression analysis in B-hSPP1 mice by ELISA. Serum was collected from wild-type mice (+/+) and homozygous B-hSPP1 mice (H/H) analyzed by ELISA with species-specific SPP1 ELISA kit. Mouse SPP1 was detectable in wild-type mice. Human SPP1 was exclusively detectable in homozygous B-hSPP1 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
The human SPP1 gene was detectable in the splenocytes of B-hSPP1 mice.Protein expression analysis:
The human SPP1 protein was detectable in the serum of B-hSPP1 mice.
