Basic Information
Strain name
C57BL/6-Spp1tm1(SPP1)/Bcgen
Common name
B-hSPP1 mice
Catalog number
111087
Background
C57BL/6N
Aliases
OPN; BNSP; BSPI; ETA-1
NCBI Gene ID
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Gene targeting strategy
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Gene targeting strategy for B-hSPP1 mice. The full coding region of mouse Spp1 gene was replaced by the full coding sequence of human SPP1 gene in B-hSPP1 mice.
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mRNA expression analysis
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Species-specific SPP1 gene expression analysis in wild-type and humanized B-hSPP1 mice by RT-PCR. Murine Spp1 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human SPP1 mRNA was exclusively detected in homozygous B-hSPP1 (H/H) mice.
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Protein expression analysis
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Species-specific SPP1 protein expression analysis in humanized B-hSPP1 mice. Serum was isolated from wild-type C57BL/6 (+/+) and homozygous B-hSPP1 (H/H) mice and analyzed using species-specific SPP1 ELISA kits. Murine SPP1 protein was detected in wild-type mice, while human SPP1 protein was exclusively detected in B-hSPP1 mice.
