Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hTDGF1 mice.
The exons 1-6 of mouse Tdgf1 gene that encode the full-length protein were replaced by human TDGF1 exons 1-6 in B-hTDGF1 mice.
-
mRNA expression analysis
-
Species-specific TDGF1 gene expression analysis in wild-type and humanized B-hTDGF1 mice by RT-PCR. Murine Tdgf1 mRNA was detected in embryos from wild-type C57BL/6 (+/+) mice, while human TDGF1 mRNA was exclusively detected in homozygous B-hTDGF1 (H/H) mice.
-
Protein expression analysis
-
Species-specific TDGF1 protein expression analysis in humanized B-hTDGF1 mice. Embryo tissue was collected from wild-type (+/+) and homozygous B-hTDGF1 (H/H) mice, and analyzed by western blot using species-specific anti-TDGF1 antibodies. TDGF1 protein was detected in wild-type and B-hTDGF1 mice due to the cross-reactivity of antibodies.
