Basic Information

Strain name
C57BL/6-Tdgf1tm1(TDGF1)/Bcgen
Common Name
B-hTDGF1 mice
Background
C57BL/6
Catalog Number
111225
Aliases
TDGF1 (CR, CR-1, CRGF, CRIPTO)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTDGF1 mice.

The exons 1-6 of mouse Tdgf1 gene that encode the full-length protein were replaced by human TDGF1 exons 1-6 in B-hTDGF1 mice.

mRNA expression analysis

Species-specific TDGF1 gene expression analysis in wild-type and humanized B-hTDGF1 mice by RT-PCR. Murine Tdgf1 mRNA was detected in embryos from wild-type C57BL/6 (+/+) mice, while human TDGF1 mRNA was exclusively detected in homozygous B-hTDGF1 (H/H) mice.

Protein expression analysis

Species-specific TDGF1 protein expression analysis in humanized B-hTDGF1 mice. Embryo tissue was collected from wild-type (+/+) and homozygous B-hTDGF1 (H/H) mice, and analyzed by western blot using species-specific anti-TDGF1 antibodies. TDGF1 protein was detected in wild-type and B-hTDGF1 mice due to the cross-reactivity of antibodies.