Basic Information
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Protein expression analysis-TSLP
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Strain specific TSLP expression analysis in wild type BALB/c mice and heterozygous B-hTSLP mice(C) by ELISA. Calcipotriol (MC903) was dissolved in ethanol and topically applied on ears of wild type BALB/c mice and heterozygous B-hTSLP mice(C) for 7 days. Ear grinding supernatant from the two strains of mouse were analyzed by ELISA. Mouse TSLP was detectable in wild type BALB/c mice (+/+) and heterozygous B-hTSLP mice(C) (H/+). Human TSLP was only detectable in heterozygous B-hTSLP mice(C) but not in wild type mice.
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Protein expression analysis-TSLPR
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Strain specific TSLPR expression analysis in heterozygous B-hTSLP/hTSLPR mice(C) by flow cytometry. Blood were collected from wild-type BALB/c mice (+/+) and heterozygous B-hTSLP/hTSLPR mice(C) (H/+;H/+), and analyzed by flow cytometry with species-specific anti-TSLPR antibody. Mouse TSLPR was detectable in wild type BALB/c mice (+/+) and heterozygous B-hTSLP/hTSLPR mice(C) (H/+). Human TSLPR was only detectable in heterozygous B-hTSLP/hTSLPR mice(C) but not in wild type mice.
