Basic Information
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Protein expression analysis
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Western blot analysis of ADORA2B protein expression in homozygous B-hADORA2B mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hADORA2B mice (H/H), and then analyzed by western blot with anti-ADORA2B antibody. 40 μg total proteins were loaded for western blotting analysis. hADORA2B was detected in heart, lung, kidney, brain, liver and small intestine from homozygous mice.
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mRNA expression analysis
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Strain specific analysis of ADORA2B mRNA expression in wild-type C57BL/6 mice and hADORA2B mice by RT-PCR. Brain RNA were isolated from wild type C57BL/6 mice (+/+) and homozygous B-hADORA2B mice (H/H). Mouse Adora2b mRNA was only detectable in wild-type C57BL/6 mice. Human ADORA2B mRNA was only detectable in homozygous B-hADORA2B mice but not in wild-type mice.
