Basic Information
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Targeting strategy
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Gene targeting strategy for B-hBDKRB2 mice.
The exons 2~3 of mouse Bdkrb2 gene that encodes the full-length protein was replaced by human BDKRB2 exons 2~3 in B-hBDKRB2 mice. -
Protein expression analysis
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Western blot analysis of BDKRB2 protein expression in homozygous B-hBDKRB2 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hBDKRB2 mice (H/H), and then analyzed by western blot with anti-BDKRB2 antibody. 1 μg/μL total proteins were loaded for western blotting analysis by JESS. BDKRB2 was detected in liver and heart in wild-type and homozygous B-hBDKRB2 mice. BDKRB2 was not detectable in brain, lung, spleen, kidney and bladder.
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mRNA expression analysis
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Strain specific analysis of BDKRB2 mRNA expression in wild-type C57BL/6 mice and B-hBDKRB2 mice by RT-PCR. Heart RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hBDKRB2 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human BDKRB2 primers. Mouse BDKRB2 mRNA was detectable in wild-type C57BL/6. Human BDKRB2 mRNA was exclusively detectable in homozygous B-hBDKRB2 mice.
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Summary
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mRNA expression analysis: Mouse BDKRB2 mRNA was detectable in the prostate of wild-type C57BL/6 mice. Human BDKRB2 mRNA was detectable in B-hBDKRB2 mice.
Protein expression analysis: BDKRB2 was detectable in liver and heart in wild-type C57BL/6 mice and homozygous B-hBDKRB2 mice, as anti-BDKRB2 antibody was cross-reactive between human and mouse.
