Basic Information
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Targeting strategy
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Gene targeting strategy for B-hC5AR2 mice. The exon 2 of mouse C5ar2 gene that encodes the full coding sequences was replaced by human C5AR2 exon 2 in B-hC5AR2 mice.
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mRNA expression analysis
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Strain specific analysis of C5AR2 gene expression in wild-type C57BL/6 mice and B-hC5AR2 mice by RT-PCR. Mouse C5ar2 mRNA was detectable in spleen of wild-type C57BL/6 mice (+/+) but not in homozygous B-hC5AR2 mice (H/H). Human C5AR2 mRNA was detectable only in homozygous B-hC5AR2 mice but not in wild-type mice.
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Protein expression analysis
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Strain specific C5AR2 expression analysis in homozygous B-hC5AR2 mice by FACS. Peritoneal washes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hC5AR2 mice (H/H) and analyzed by FACS with anti-C5AR2 antibody. C5AR2 was both detectable in wild-type mice and homozygous B-hC5AR2 mice with anti-mC5AR2 antibody. Anti-mC5AR2 antibody was cross-reactive between mouse and human (data not shown).
