Basic Information
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Description
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- CMKLR1 also known as ChemR23, is the receptor for the chemoattractant—chemerin.
- The exon 3 of mouse Cmklr1 gene that encodes the full-length protein was replaced by human CMKLR1 exons 3-4 in B-hCMKLR1 mice.
- CMKLR1 protein was detected in spleen, blood, lymph node and bone marrow of homozygous B-hCMKLR1 mice
- Application: This product is used for pharmacodynamics and safety evaluation of anti-inflammatory drugs.
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Targeting strategy
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Gene targeting strategy for B-hCMKLR1 mice. The exon 3 of mouse Cmklr1 gene that encodes the full-length protein was replaced by human CMKLR1 exons 3-4 in B-hCMKLR1 mice.
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mRNA expression analysis
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Strain specific analysis of CMKLR1 mRNA expression in wild-type C57BL/6 mice and B-hCMKLR1 mice by RT-PCR. Spleen RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human CMKLR1 primers. Mouse Cmklr1 mRNA was detectable in wild-type C57BL/6 mice. Human CMKLR1 mRNA was detectable only in homozygous B-hCMKLR1 mice but not in wild-type mice. Specially, the human CMKLR1 isoform b was actually transcribed in B-hCMKLR1 mice.
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Protein expression analysis
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Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes, blood, lymph modes (LN) and bone marrow (BM) were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CMKLR1 antibody (Mouse ChemR23 PE-conjugated Antibody, R&D, Cat FAB7610P; Rat IgG2B PE-conjugated Antibody, R&D, IC013P). mCMKLR1 was detectable in wild-type mice, hCMKLR1 was detectable in homozygous B-hCMKLR1 mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes, blood and bone marrow (BM) were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and analyzed by flow cytometry with species-specific anti-CMKLR1 antibody (Mouse ChemR23 PE-conjugated Antibody, R&D, Cat FAB7610P; Rat IgG2B PE-conjugated Antibody, R&D, IC013P). mCMKLR1 was detectable in wild-type mice, hCMKLR1 was detectable in homozygous B-hCMKLR1 mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes and blood cells were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and protein expression was analyzed with anti-human CMKLR1 antibody (R&D, 84939) by flow cytometry. hCMKLR1 was detectable in T cells, B cells and NK cells of homozygous B-hCMKLR1 mice and wild-type C57BL/6N mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes and blood cells were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and protein expression was analyzed with anti-human CMKLR1 antibody (R&D, 84939) by flow cytometry. hCMKLR1 was detectable in monocytes and macrophages of homozygous B-hCMKLR1 mice and wild-type C57BL/6N mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Splenocytes and blood cells were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and protein expression was analyzed with anti-human CMKLR1 antibody (R&D, 84939) by flow cytometry. hCMKLR1 was detectable in neutrophils and DCs of homozygous B-hCMKLR1 mice and wild-type C57BL/6N mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Lymph nodes and bone marrow were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and protein expression was analyzed with anti-human CMKLR1 antibody (R&D, 84939) by flow cytometry. hCMKLR1 was detectable in T cells, B cells and NK cells of homozygous B-hCMKLR1 mice and wild-type C57BL/6N mice.
Strain specific CMKLR1 expression analysis in homozygous B-hCMKLR1 mice by flow cytometry. Bone marrow was collected from wild-type C57BL/6N mice (+/+) and homozygous B-hCMKLR1 mice (H/H), and protein expression was analyzed with anti-human CMKLR1 antibody (R&D, 84939) by flow cytometry. hCMKLR1 was detectable in monocytes, macrophages, neutrophils and DCs of homozygous B-hCMKLR1 mice and wild-type C57BL/6N mice.
