Basic Information

Strain Name
C57BL/6-Cxcr5tm1(CXCR5)/Bcgen
Stock Number
110970
Common Name
B-hCXCR5 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Aliases
CXCR5(C-X-C motif chemokine receptor 5), BLR1, CD185, MDR15
Species
C57BL/6
Appearance
Black
Genotypes
Heterozygous
NCBI Gene ID

Targeting strategy

Targeting strategy

Gene targeting strategy for B-hCXCR5 mice. The exons 1~2 of mouse Cxcr5 gene that encode the full-length protein were replaced by human CXCR5 exons 1~2 in B-hCXCR5 mice.

Details

Protein expression analysis

Strain specific CXCR5 expression analysis in heterozygous B-hCXCR5 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCXCR5 (H/+) mice, and analyzed by flow cytometry with species-specific CD24 antibody. Mouse CXCR5 was detectable in WT mice and heterozygous B-hCXCR5. Human CXCR5 was exclusively detectable in heterozygous B-hCXCR5 but not WT mice.