mRNA and protein expression analysis
Strain specific analysis of ETAR gene expression in B-ETAR mice by RT-PCR and flow cytometry. A. Mouse Ednra mRNA was detectable in lung of C57BL/6 (+/+) mice. Human ETAR mRNA was detectable only in homozygous B-hETAR mice (H/H) but not in wild-type mice. B. Monocytes and macrophages of bone marrow from C57BL/6 and B-hETAR mice (H/H) were analyzed by flow cytometry with anti-ETAR antibodies. ETAR was detectable in C57BL/6 and homozygous B-hETAR mice. This anti-mouse ETAR antibody also cross reacts with human ETAR. Taken together with RT results, B-hETAR mice successfully express human ETAR protein.