Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFSHR mice.
The exon 2 of mouse Fshr gene that encode the full-length protein was replaced by human FSHR exon 2 in B-hFSHR mice. -
mRNA expression analysis
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Strain specific analysis of FSHR gene expression in wild-type C57BL/6 mice and B-hFSHR mice by RT-PCR. Mouse Fshr mRNA was detectable in ovary and testis of wild-type C57BL/6 mice (+/+). Human FSHR mRNA was detectable only in homozygous B-hFSHR mice (H/H) but not in wild-type mice.
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Protein expression analysis
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Strain specific FSHR expression analysis in homozygous B-hFSHR mice by western blot. Ovary, testis were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFSHR mice (H/H), and analyzed by western blot with anti-FSHR antibody. FSHR was detectable in wild type mice and homozygous B-hFSHR mice, as the antibody is crossly reactive with FSHR in human and mice. FSHR was not detectable in FSHR KO mice.
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Summary
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mRNA expression analysis:
Mouse FSHR mRNA was detectable in wild-type (+/+). Human FSHR mRNA was detectable in B-hFSHR mice (H/H) mice but not in wild-type mice.Protein expression analysis:
FSHR was detectable in homozygous B-hFSHR.
