Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFXI mice.
The exons 1~15 of mouse FXI gene that encode full-length protein were replaced by the exons 1~15 of human FXI gene in B-hFXI mice.
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mRNA expression analysis in humanized B-hFXI mice
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Strain specific analysis of FXI mRNA expression in wild-type C57BL/6 mice and B-hFXI mice by RT-PCR. Liver RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hFXI mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FXI primers. Mouse FXI mRNA were detectable in wild-type C57BL/6 mice. Human FXI mRNA was only detectable in homozygous B-hFXI mice.
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Protein expression analysis in humanized B-hFXI mice
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Strain specific FXI expression analysis in wild-type C57BL/6 mice and homozygous humanized B-hFXI mice by ELISA. Serum was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hFXI mice (H/H) (n=2). Protein expression level of FXI was analyzed by ELISA. Human FXI was exclusively detectable in homozygous B-hFXI mice. Values are expressed as mean ± SEM. Significance was determined by unpaired t-test. *P < 0.05, **P < 0.01, ***p < 0.0001.
