Basic Information
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Targeting strategy
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Gene targeting strategy for B-hFZD7 mice.
The exon of mouse Fzd7 gene that encodes the full-length protein was replaced by human FZD7 exon in B-hFZD7 mice. -
Protein expression analysis
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Western blot analysis of FZD7 protein expression in homozygous B-hFZD7 mice. Various tissue lysates were collected from wild-type C57BL/6 (+/+) mice and homozygous B-hFZD7 (H/H) mice, and then analyzed by western blot with anti-FZD7 antibody. 40 μg total proteins were loaded for western blotting analysis. FZD7 was detected in heart, brain and skeletal muscle. The antibody cross recognises human and mouse FZD7.
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mRNA expression analysis
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Strain specific analysis of FZD7 mRNA expression in wild-type C57BL/6 mice and B-hFZD7 mice by RT-PCR. Brain RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hFZD7 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human FZD7 primers. Mouse Fzd7 mRNA was detectable only in wild-type C57BL/6 mice. Human FZD7 mRNA was detectable only in homozygous B-hFZD7 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Fzd7 mRNA was detectable in the brain of wild-type C57BL/6 mice. Human FZD7 mRNA was detectable in B-hFZD7 mice.Protein expression analysis:
Human and mouse FZD7 proteins were both detectable in wild-type C57BL/6 and B-hFZD7 mice. The antibody cross recognised human and mouse FZD7.
