Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGPR40 mice.
The exon 1 of mouse Gpr40 gene that encodes the full-length protein was replaced by human GPR40 exon 1 in B-hGPR40 mice.
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Protein expression analysis
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Western blot analysis of GPR40 protein expression in homozygous B-hGPR40 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hGPR40 mice (H/H), and then analyzed by western blot with species-specific anti-GPR40 antibody. 20 μg total proteins were loaded for western blotting analysis. hGPR40 was detected in pancreas from homozygous mice but not in liver,kidney and stomach.
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mRNA expression analysis
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Strain specific analysis of GPR40 mRNA expression in wild-type C57BL/6 mice and B-hGPR40 mice by RT-PCR. BM RNA was isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hGPR40 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR40 primers. Mouse Gpr40 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR40 mRNA was detectable only in homozygous B-hGPR40 mice but not in wild-type mice.
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Summary
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- mRNA expression analysis:
Mouse Gpr40 mRNA was detectable in BM of wild-type C57BL/6 mice. Human GPR40 mRNA was detectable in B-hGPR40 mice.
- Protein expression analysis:
Human GPR40 was detectable in pancreas of homozygous B-hGPR40 mice.
