Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGPR75 mice.
The exons 2 of mouse Gpr75 gene that encode the full-length protein was replaced by human GPR75 exons 2 in B-hGPR75 mice. -
mRNA expression analysis
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Strain specific analysis of GPR75 gene expression in wild-type C57BL/6 mice and B-hGPR75 mice by RT-PCR. Mouse GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice, GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice and homozygous B-hGPR75 mice (H/H) due to the high genetic similarity, and we sequenced and confirmed that the replaced region of homozygous B-hGPR75 mice (H/H) is the human GPR75 sequence.
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Summary
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mRNA expression analysis:
Mouse GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice, GPR75 mRNA was detectable in brain of wild-type C57BL/6 mice and homozygous B-hGPR75 mice (H/H) due to the high genetic similarity, and we sequenced and confirmed that the replaced region of homozygous B-hGPR75 mice (H/H) is the human GPR75 sequence.
