Basic Information
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Targeting strategy
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Gene targeting strategy for B-hGPR87 mice.
The exons 2~3 of mouse Gpr87 gene that encode the full-length protein were replaced by human GPR87 exons 2~3 in B-hGPR87 mice.
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mRNA expression analysis
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Strain specific analysis of GPR87 mRNA expression in wild-type C57BL/6 mice and B-hGPR87 mice by RT-PCR. Lung RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hGPR87 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human GPR87 primers. Mouse Gpr87 mRNA was detectable only in wild-type C57BL/6 mice. Human GPR87 mRNA was detectable only in homozygous B-hGPR87 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of GPR87 protein expression in homozygous B-hGPR87 mice. Various tissue lysates were collected from C57BL/6N wildtype (+/+) mice and homozygous B-hGPR87 (H/H) mice, and then analyzed by western blot with anti-GPR87 antibody. 40 μg total proteins were loaded for western blotting analysis. GPR87 was detected in stomach, lung, esophagus and bladder.
