Basic Information

Strain Name
C57BL/6-Lgr4tm1(LGR4)Bcgen/Bcgen
Common Name
B-hLGR4 mice
Background
C57BL/6
Catalog number
110968
Aliases
DPSL, GPR48, BNMD17
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hLGR4 mice. The exons 5~18 of mouse Lgr4 gene were replaced by human LGR4 exons 5~18 in B-hLGR4 mice.

Protein expression analysis

Western blot analysis of LGR4 protein expression in homozygous B-hLGR4 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hLGR4 mice (H/H), and then analyzed by western blot with anti-LGR4 antibody. 60 μg total proteins were loaded for western blotting analysis. LGR4 protein was detected in the lung, testis, colon, spleen, kidney of wild-type C57BL/6 mice and homozygous B-hLGR4 mice, as the antibody is cross-recognize both human and mouse LGR4.

mRNA expression analysis

Strain specific analysis of LGR4 mRNA expression in wild-type C57BL/6 mice and B-hLGR4 mice by RT-PCR. Kidney RNA were isolated from wildtype C57BL/6 mice (+/+) and homozygous B-hLGR4 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human LGR4 primers. Mouse LGR4 mRNA was detected in wild-type C57BL/6 mice. Human LGR4 mRNA was detected in homozygous B-hLGR4 mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Lgr4 mRNA was detected in kidney of wild-type C57BL/6 mice. Human LGR4 mRNA was detected in kidney of B-hLGR4 mice.

Protein expression analysis:

LGR4 protein was detected in the lung, testis, colon, spleen, kidney of wild-type C57BL/6 mice and homozygous B-hLGR4 mice, as the antibody is cross-recognize both human and mouse LGR4.

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