Basic Information
Strain Name
C57BL/6N-Mc4rtm1(MC4R)Bcgen/Bcgen
Stock Number
112592
Common Name
B-hMC4R mice
Aliases
MC4R: BMIQ20
Background
C57BL/6N
NCBI Gene ID
-
Targeting strategy
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Gene targeting strategy for B-hMC4R mice.
Exon 1 of the mouse Mc4r gene, which encodes the full-length protein, was replaced by human MC4R exon 1 in B-hMC4R mice.
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mRNA expression analysis
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Strain specific analysis of MC4R mRNA expression in wild-type C57BL/6N mice and B-hMC4R mice by RT-PCR. Brain RNA was isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hMC4R mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human MC4R primers. Mouse Mc4r mRNA was detectable only in wild-type C57BL/6N mice. Human MC4R mRNA was detectable only in homozygous B-hMC4R mice but not in wild-type mice.
