Basic Information
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mRNA expression analysis
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Strain specific analysis of SUCNR1 mRNA expression in wild-type C57BL/6N mice and B-hSUCNR1 mice by RT-PCR. Liver RNA was isolated from wild-type C57BL/6N mice (+/+) and homozygous B-hSUCNR1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human SUCNR1 primers. Mouse Sucnr1 mRNA was detectable only in wild-type C57BL/6N mice. Human SUCNR1 mRNA was detectable in homozygous B-hSUCNR1 mice but not in wild-type mice.
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Protein expression analysis
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Western blot analysis of SUCNR1 protein expression in homozygous B-hSUCNR1 mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hSUCNR1 mice (H/H), and then analyzed by western blot with anti-SUCNR1 antibody. 4 μg total protein was loaded for western blotting analysis. SUCNR1 protein was detected in the spleen, liver and kidney of wild-type C57BL/6N mice and homozygous B-SUCNR1 mice, as the antibody recognizes both human and mouse SUCNR1.
