Basic Information
VPAC1:VIPR1, II, HVR1, RDC1, V1RG, VIPR, VIRG, VAPC1, VPAC1, VPAC1R, VIP-R-1, VPCAP1R, PACAP-R2, PACAP-R-2
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mRNA expression analysis
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Strain specific analysis of VPAC1 mRNA expression in wild-type C57BL/6 mice and B-hVPAC1 mice by RT-PCR. Small intestine RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hVPAC1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human VPAC1 primers. Mouse Vpac1 mRNA was detectable only in wild-type C57BL/6 mice. Human VPAC1 mRNA was detectable only in homozygous B-hVPAC1 mice.
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Protein expression analysis
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Western blot analysis of VPAC1 protein expression in homozygous B-hVPAC1 mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hVPAC1 mice (H/H), and then analyzed by western blot with anti-VPAC1 antibody. 30 μg total proteins were loaded for western blotting analysis. VPAC1 was detected in lung and small intestine of wild-type and homozygous mice, as anti-VPAC1 antibody was cross-reactive between human and mouse.
