Basic Information
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Targeting strategy
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Gene targeting strategy for B-hBAMBI mice.
The exons 1~3 of mouse Bambi gene that encode the full-length protein were replaced by human BAMBI exons 1~3 in B-hBAMBI mice.
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Protein expression analysis
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Western blot analysis of BAMBI protein expression in homozygous B-hBAMBI mice. Various tissue lysates were collected from wild-type C57BL/6N mice (+/+) and homozygous B-hBAMBI mice (H/H), and then analyzed by western blot with anti-BAMBI antibody. 40 μg total proteins were loaded for western blotting analysis. BAMBI was detected in lung, heart, liver, kidney and ovary. (There was no β-actin in the heart tissue)
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mRNA expression analysis
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Strain specific analysis of BAMBI mRNA expression in wild-type C57BL/6 mice and B-hBAMBI mice by RT-PCR. Heart RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hBAMBI mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human BAMBI primers. Mouse Bambi mRNA was detectable only in wild-type C57BL/6 mice. Human BAMBI mRNA was detectable only in homozygous B-hBAMBI mice but not in wild-type mice.
