Basic Information

Strain name
C57BL/6-Btlatm1(BTLA)Tnfrsf14tm1(TNFRSF14)/Bcgen
Common Name
B-hBTLA/hHVEM mice
Background
C57BL/6N
Catalog Number
121080
Aliases
BTLA, BTLA1, CD272, B and T lymphocyte associated; TNFRSF14, HVEM, ATAR, CD270, HVEA, HVEM, LIGHTR, TR2, Herpesvirus entry mediator, TNF receptor superfamily member 14
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hBTLA/hHVEM mice.

The exon 2 of mouse Btla gene that encodes the extracellular domain was replaced by human BTLA exon 2 in B-hBTLA/hHVEM mice. The exons 1~6 of mouse Tnfrsf14 gene that encode the extracellular domain were replaced by human TNFRSF14 exons 1~6 in B-hBTLA/hHVEM mice.

mRNA expression analysis

Strain specific analysis of BTLA and HVEM gene expression in wild type (WT) mice and B-hBTLA/hHVEM mice by RT-PCR.

Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).

Protein expression analysis in B cells

Strain specific BTLA  expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-BTLA antibody. Mouse BTLA  was detectable in WT mice (+/+). Human BTLA was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).

Protein expression analysis in T cells

Strain specific HVEM  expression analysis in homozygous B-hBTLA/hHVEM mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hBTLA/hHVEM mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-HVEM antibody. Mouse HVEM  was detectable in WT mice (+/+). Human HVEM was exclusively detectable in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).

Summary

mRNA expression analysis:

Mouse Btla and Hvem mRNA was detectable only in splenocytes of WT mice (+/+). Human BTLA and HVEM mRNA was detectable only in homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+).

Protein expression analysis:

Human BTLA was exclusively detectable on B cells of homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+), and mouse BTLA was detectable only in WT mice (+/+).

Human HVEM was exclusively detectable on T cells of homozygous B-hBTLA/hHVEM mice (H/H;H/H) but not in WT mice (+/+), and mouse HVEM was detectable only in WT mice (+/+).