Basic Information

Strain name
C57BL/6-C5ar1tm1(hC5AR1)/Bcgen
Common name
B-hC5AR1 mice
Background
C57BL/6
Catalog number
110078
Related genes
C5A; C5AR; C5R1; CD88

Description

The receptor for C5a plays a critical role in numerous inflammatory conditions. C5a is a member of the complement cascade, an important mechanism for host defense against bacteria. Increased complement activation and excessive production of C5a have been implicated in the pathogenesis of several inflammatory diseases, and considerable effort has gone into developing C5aR antagonists, including organic small molecules and peptide antagonists. As one of the most potent mediators of inflammation, the complement component C5a is a promising target for anti-inflammatory drugs. However, a drawback of using animal models to develop antagonists of complement activation is that interspecies differences in receptors and ligands may complicate drug development and delay the evaluation of their efficacy in humans.  We reasoned that the availability of human C5aR knock-in mice might provide an effective means to validate this receptor in mouse models of disease, using reagents that could be developed for use in clinical trials. Details

Details

Protein expression analysis(spleen)

Strain specific C5AR1 expression analysis in homozygous B-hC5AR1 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. C5AR1 was detectable in mouse spleen Dendritic cells, granulocytes and macrophages. Mouse C5AR1 was detectable in WT mice. Human C5AR1 was exclusively detectable in homozygous B-hC5AR1 but not WT mice.

Protein expression analysis(Bone marrow)

Strain specific C5AR1 expression analysis in homozygous B-hC5AR1 mice by flow cytometry. Bone marrow were collected from WT and homozygous B-hC5AR1 (H/H) mice, and analyzed by flow cytometry with species-specific anti-C5AR1 antibody. Mouse C5AR1 was detectable in WT mice. Human C5AR1 was exclusively detectable in homozygous B-hC5AR1 dendritic cells but not WT mice.

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