Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD16A mice(C). The regulatory region and exons 1~5 of mouse Fcgr4 gene that encodes the full-length protein were replaced by human FCGR3A exon 1~ intron 3 genome sequence plus exons 4~6 CDS carrying F158V mutation and regulatory region in B-hCD16A mice(C).
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Protein expression analysis in blood and spleen-NK cells
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Strain specific CD16A expression analysis in homozygous B-hCD16A mice(C) by flow cytometry. Blood and spleen were collected from wild-type mice (+/+) and homozygous B-hCD16A mice(C) (H/H), and analyzed by flow cytometry with species-specific anti-CD16A antibody. Mouse CD16A was not detectable in wild-type mice. Human CD16A was only detectable in homozygous B-hCD16A mice(C) blood(A) and spleen(B).
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Analysis of spleen leukocyte subpopulations
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Analysis of spleen leukocyte subpopulations. Splenocytes were isolated from female BALB/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
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Analysis of spleen T cell subpopulations
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Analysis of spleen T cell subpopulations. Splenocytes were isolated from female BABL/c and B-hCD16A mice(C) (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Values are expressed as mean ± SEM.
