Basic Information

Strain name
CB-17 SCID-Fcgr4tm2(FCGR3A)/Bcgen
Common name
B-hCD16A mice(CB-17 SCID)
Background
CB-17 SCID
Catalog Number
111174
NCBI Gene ID
Related Gene
FCGR3A (Fc fragment of IgG receptor IIIa), CD16A

Targeting Strategy

Gene targeting strategy for B-hCD16A mice(CB-17 SCID).

The regulatory region and exons 1~5 of mouse Fcgr4 gene that encode the full-length protein were replaced by human FCGR3A exons 1~4 and regulatory region in B-hCD16A mice(CB-17 SCID).

Surface Expression in NK Cells

Species-specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID) by flow cytometry.  Splenocytes were collected from wild-type CB-17 SCID mice(+/+)  and homozygous B-hFCGR3A mice(CB-17 SCID) (H/H). Mouse FCGRIV was not detectable in NK cells of wild-type CB-17 SCID mice. Human FCGR3A was detectable in NK cells of B-hFCGR3A mice(CB-17 SCID).

Surface Expression in Granulocytes

Species-specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID)  by flow cytometry.  Splenocytes were collected from wild-type CB-17 SCID mice(+/+)  and homozygous B-hFCGR3A mice (CB-17 SCID) (H/H). Mouse FCGR3A was exclusively detectable in granulocytes of wild-type CB-17 SCID. Human FCGR3A was not detectable in granulocytes of wild-type CB-17 SCID mice and homozygous B-hFCGR3A mice (CB-17 SCID).

Surface Expression in Macrophages

Strain specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID)  by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type CB-17 SCID mice(+/+)  and homozygous B-hFCGR3A mice (CB-17 SCID) (H/H). Mouse FCGRIV was exclusively detectable in PEMs of wild-type C57BL/6 mice. Human FCGR3A was exclusively detectable in PEMs of homozygous B-hFCGR3A mice (CB-17 SCID).