Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hCD16A mice(CB-17 SCID).
The regulatory region and exons 1~5 of mouse Fcgr4 gene that encode the full-length protein were replaced by human FCGR3A exons 1~4 and regulatory region in B-hCD16A mice(CB-17 SCID).
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Surface Expression in NK Cells
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Species-specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice(+/+) and homozygous B-hFCGR3A mice(CB-17 SCID) (H/H). Mouse FCGRIV was not detectable in NK cells of wild-type CB-17 SCID mice. Human FCGR3A was detectable in NK cells of B-hFCGR3A mice(CB-17 SCID).
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Surface Expression in Granulocytes
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Species-specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID) by flow cytometry. Splenocytes were collected from wild-type CB-17 SCID mice(+/+) and homozygous B-hFCGR3A mice (CB-17 SCID) (H/H). Mouse FCGR3A was exclusively detectable in granulocytes of wild-type CB-17 SCID. Human FCGR3A was not detectable in granulocytes of wild-type CB-17 SCID mice and homozygous B-hFCGR3A mice (CB-17 SCID).
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Surface Expression in Macrophages
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Strain specific FCGR3A expression analysis in wild-type CB-17 SCID mice and homozygous B-hFCGR3A(CB-17 SCID) by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type CB-17 SCID mice(+/+) and homozygous B-hFCGR3A mice (CB-17 SCID) (H/H). Mouse FCGRIV was exclusively detectable in PEMs of wild-type C57BL/6 mice. Human FCGR3A was exclusively detectable in PEMs of homozygous B-hFCGR3A mice (CB-17 SCID).
