Basic Information

Strain Name
C57BL/6-Igs2tm1(FCGR3B)Bcgen/Bcgen
Common Name
B-hCD16B mice
Background
C57BL/6
Catalog Number
111863
NCBI Gene ID
Related Genes
CD16, CD16b, FCRIIIb

Targeting strategy

Gene targeting strategy for B-hCD16B mice. The human gene coding sequence was inserted to mouse Hipp11(H11) allele in B-hCD16B mice.

Protein expression analysis in blood and spleen granulocytes

Strain specific CD16B expression analysis in homozygous B-hCD16B mice by flow cytometry. Blood (A) and spleen (B) were collected from wild-type mice (+/+) and homozygous B-hCD16B mice (H/H), and analyzed by flow cytometry with species-specific anti-CD16 antibody, respectively. Human CD16B was exclusively detectable in granulocytes of homozygous B-hCD16B mice. Note: the clone of anti-human CD16B antibody is 3G8.

Analysis of leukocytes cell subpopulation in spleen

Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.

Analysis of T cell subpopulation in spleen

Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.

Analysis of leukocytes cell subpopulation in blood

Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and homozygous B-hCD16B mice (n=3, 7-week-old). Flow cytometry analysis of the blood was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hCD16B mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.

Summary

Protein expression analysis:
Human CD16B was exclusively detectable in homozygous B-hCD16B mice but not wild-type mice.

Leukocytes profile:
Percentages of T, B, NK cells, monocyte/macrophages, and DC were similar in homozygous B-hCD16B mice and wild-type mice, demonstrating that introduction of hCD16B does not change the overall development, differentiation, or distribution of these cell types in spleen, lymph node leukocytes and blood.

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