Basic Information

Strain Name
C57BL/6-Cd27tm1(CD27)/Bcgen
Stock Number
110006
Common Name
B-hCD27 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Cd27 (CD27 antigen)
Species
C57BL/6J
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD27 mice. The exons 1-5 of mouse Cd27 gene that encode the extracellular domain were replaced by human CD27 exons 1-5 in B-hCD27 mice.

Protein expression analysis

Species-specific CD27 protein expression analysis in humanized B-hCD27 mice. Following anti-CD3ε stimulation in vivo, splenocytes were isolated from wild-type (WT) and homozygous B-hCD27 mice and analyzed by flow cytometry using species-specific anti-CD27 antibodies. Murine CD27 protein was detected in wild-type and B-hCD27 mice, due to the cross-reactivity of the antibody, while human CD27 protein was exclusively detected in B-hCD27 mice.

In vivo efficacy of anti-human CD27 antibodies

Antitumor activity of an anti-human CD27 antibody in humanized B-hCD27 mice. Murine colon cancer MC38 cells (5×105) were subcutaneously implanted into heterozygous B-hCD27 mice (male, 4-6 week-old, n=5). Mice were grouped when the tumor volume reached approximately 100 mm3, at which time they were treated with either a vehicle control or an anti-human CD27 antibody at doses and schedules as indicated. (A) The anti-human CD27 antibody inhibited MC38 tumor growth in B-hCD27 mice, (B) without negatively impacting body weight changes during treatment. B-hCD27 mice provide a powerful preclinical model for in vivo evaluation of anti-human CD27 antibodies. Values are expressed as mean ± SEM.

 

B-hCD27-mice-details-Human-CD27-mAb-efficacy-evaluation-MC38-cell-line

Antitumor activity of an anti-human CD27 antibody in humanized B-hCD27 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B- hCD27 mice. Mice were divided into control and treatment groups (n=5) when the tumor size was approximately 150±50 mm3. Anti-hCD27 antibody (varlilumab) significantly inhibited tumor growth in homozygous B-hCD27 mice, suggesting that the B-hCD27 mouse model is an effective tool for in vivo hCD27 antibody efficacy studies. (A) Tumor average volume ± SEM, (B) Mice average body weight ± SEM.

References

1. J Immunol. 2013 Oct 15;191(8):4174-83. doi: 10.4049/jimmunol.1300409. Epub 2013 Sep 11.
2. Blood. 2017 Jul 20;130(3):297-309. doi: 10.1182/blood-2016-12-756585. Epub 2017 May 11.
3. J Clin Oncol. 2017 Jun 20;35(18):2028-2036. doi: 10.1200/JCO.2016.70.1508. Epub 2017 May 2.
4. Immunotherapy. 2015;7(6):655-67. doi: 10.2217/imt.15.32. Epub 2015 Jun 22.