Basic Information

Strain name
Common name
B-hCD28/hB7-H3 mice
Background
C57BL/6
Catalog number
121251
NCBI Gene ID

Gene Targeting Strategy

Exons 2-3 of the murine Cd28 gene, which encodes the extracellular domain, were replaced by exons 2-3 of the human CD28 gene in B-hCD28/hB7-H3 mice. Exons 3~4 of the murine Cd276 gene, which encodes the extracellular domain, were replaced by exons 3~6 of the human CD276 gene in B-hCD28/hB7-H3 mice.

Protein Expression Analysis

Species-specific CD28 expression analysis in double humanized B-hCD28/hB7-H3 mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hCD28/hB7-H3 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD28 antibodies. Human CD28 was exclusively detected in B-hCD28/hB7-H3 mice compared to wild-type mice.

 

Species-specific B7-H3 expression analysis in double humanized B-hCD28/hB7-H3 mice. Epididymis was collected from wild-type C57BL/6 (+/+) and homozygous B-hCD28/hB7-H3 (H/H) mice and analyzed by western blot using anti-B7-H3 antibodies. B7-H3 was detected in wild-type and B-hCD28/hB7-H3 mice due to the cross-reactivity of the antibodies.