Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD30 mice.
The exons 1~9 of mouse Cd30 gene that encode the extracellular domain were replaced by human CD30 exons 1~11 in B-hCD30 mice.
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Protein expression analysis in T cells
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Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.
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Protein expression analysis in NK cells
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Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.
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mRNA expression analysis
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Strain specific analysis of CD30 mRNA expression in wild-type C57BL/6 mice and B-hCD30 mice by RT-PCR. Mouse Cd30 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice. Human CD30 mRNA was detectable only in homozygous B-hCD30 mice but not in wild-type mice.
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Summary
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mRNA expression analysis:
Mouse Cd30 mRNA was detectable in spleen of wild-type C57BL/6 mice. Human CD30 mRNA was detectable in B-hCD30 mice.
Protein expression analysis:
Human CD30 was exclusively detectable on T cells and NK cells in spleen of homozygous B-hCD30 mice, and mouse CD30 was detectable only in wild-type C57BL/6 mice.
