Basic Information

Strain Name
C57BL/6-Cd30tm1(CD30)Bcgen/Bcgen
Common Name
B-hCD30 mice
Background
C57BL/6
Catalog number
110888
Aliases
TNFRSF8, Ki-1, D1S166E
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD30 mice. The exons 1~9 of mouse Cd30 gene that encode the extracellular domain were replaced by human CD30 exons 1~11 in B-hCD30 mice.

Protein expression analysis in T cells

Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.

Protein expression analysis in NK cells

Strain specific CD30 expression analysis in homozygous B-hCD30 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD30 mice (H/H) stimulated with PMA/Ionomycin in vitro, and analyzed by flow cytometry with anti-CD30 antibody. Anti-mouse CD30 antibody was cross-reactive between mouse and human. Combined with mRNA expression in wild-type and homozygous B-hCD30 mice, it can be proved that mouse CD30 was detectable in wild-type mice. HCD30 was exclusively detectable in the homozygous mice.

mRNA expression analysis

Strain specific analysis of CD30 mRNA expression in wild-type C57BL/6 mice and B-hCD30 mice by RT-PCR. Mouse Cd30 mRNA was detectable only in splenocytes of wild-type C57BL/6 mice. Human CD30 mRNA was detectable only in homozygous B-hCD30 mice but not in wild-type mice.

Summary

mRNA expression analysis:

Mouse Cd30 mRNA was detectable in spleen of wild-type C57BL/6 mice. Human CD30 mRNA was detectable in B-hCD30 mice.

Protein expression analysis:

Human CD30 was exclusively  detectable on T cells and NK cells in spleen of homozygous B-hCD30 mice, and mouse CD30 was detectable only in wild-type C57BL/6 mice.

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