Basic Information
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Protein expression analysis of splenic and blood T cells
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), respectively, and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was not detectable in T cells of wild-type C57BL/6 mice. hCD32A was not detectable in T cells of homozygous B-hCD32A mice.
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Protein expression analysis of splenic and blood B cells
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was not detectable in B cells of wild-type C57BL/6 mice. hCD32A was not detectable in B cells of homozygous B-hCD32A mice.
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Protein expression analysis in splenic and blood NK cells
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was detectable in NK cells of wild-type C57BL/6 mice. hCD32A was not detectable in NK cells of homozygous B-hCD32A mice.
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Protein expression analysis in splenic and blood DC cells
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was detectable in DC cells of wild-type C57BL/6 mice. hCD32A was detectable in DC cells of homozygous B-hCD32A mice.
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Protein expression analysis in splenic and blood monocytes
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was detectable in monocytes of wild-type C57BL/6 mice. hCD32A was weakly detectable in monocytes of homozygous B-hCD32A mice.
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Protein expression analysis in splenic and blood macrophages
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was detectable in macrophages of wild-type C57BL/6 mice. hCD32A was detectable in macrophages of homozygous B-hCD32A mice.
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Protein expression analysis in splenic and blood neutrophils
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Strain specific CD32A expression analysis in wild type C57BL/6 mice and homozygous humanized B-hCD32A mice by flow cytometry. Splenocytes (A) and blood (B) were collected from wild type C57BL/6 mice (+/+) and homozygous B-hCD32A mice (H/H), and analyzed by flow cytometry with species-specific anti-mCD16 antibody and anti-hCD32A antibody. mCD16 was detectable in neutrophils of wild-type C57BL/6 mice. hCD32A was detectable in neutrophils of homozygous B-hCD32A mice.
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Frequency of leukocyte subpopulations in spleen of humanized B-hCD32A mice
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Frequency of leukocyte subpopulations in spleen by flow cytometry. Splenocytes were isolated from wild type C57BL/6 mice and homozygous B-hCD32A mice (n=3, 7-week-old). A. Flow cytometry analysis of the splenocytes was performed to assess the frequency of leukocyte subpopulations. B. Frequency of T cell subpopulations. Percentages of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes, macrophages, CD4+ T cells, CD8+ T cells and Tregs in B-hCD32A mice were similar to those in C57BL/6 mice, demonstrating that humanization of CD32A does not change the frequency or distribution of these cell types in spleen. The frequency of leukocyte subpopulations in lymph nodes and blood of B-Hc d32a mice were also comparable to wild-type C57BL/6 mice (Data not shown). Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***p < 0.0001.
