Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD3E/hCD38 mice.
The exons 2-6 of mouse Cd3e gene that encode the extracellular domain were replaced by human CD3E exons 2-7 in B-hCD3E/hCD38 mice. The CDS of human CD38 encoding the extracellular domain was inserted following partial exon 2 of mouse Cd38 in B-hCD3E/hCD38 mice.
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Protein expression analysis in T cells
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Strain specific CD3E expression analysis in homozygous B-hCD3E/hCD38 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hCD38 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD3ε antibody. Mouse CD3E was detectable in WT mice (+/+). Human CD3E was exclusively detectable in homozygous B-hCD3E/hCD38 mice (H/H;H/H) but not in WT mice (+/+).
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Protein expression analysis in B cells
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Strain specific CD38 expression analysis in homozygous B-hCD3E/hCD38 mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD3E/hCD38 mice (H/H;H/H), and analyzed by flow cytometry with species-specific anti-CD38 antibody. Mouse CD38 was detectable in WT mice (+/+). Human CD38 was exclusively detectable in homozygous B-hCD3E/hCD38 mice (H/H;H/H) but not in WT mice (+/+).
