CD47 is an integrin associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation and immunity. CD47 is an important “self” mark on the cell surface and inhibits macrophagocytosis by interaction with SIRPα on macrophage surface. Animal studies have shown that a CD47 antibody is an effective treatment for multiple types of tumors. CD47 is another target for tumor immunity following PD-1/CD47.
mRNA expression analysis
Strain specific analysis of CD47 gene expression in WT and B-hCD47 mice(C) by RT-PCR. Mouse Cd47 mRNA was only detectable in splenocytes of wild-type (+/+) mice. Human CD47 mRNA was only detectable in H/H but not in +/+ mice.
Protein expression analysis
Strain specific CD47 expression analysis in homozygous B-hCD47 mice(C) by flow cytometry.
Splenocytes were collected from WT and homozygous B-hCD47 (H/H) mice stimulated with anti-CD3ε in vivo and analyzed by flow cytometry with species-specific anti-CD47 antibody. Mouse CD47 was only detectable in WT mice. Human CD47 was exclusively detectable in homozygous B-hCD47 mice but not in WT mice.