Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCTLA4 mice(C).
The exon 2 of mouse Ctla4 gene that encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4 mice(C). -
Protein expression analysis
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Strain specific CTLA4 protein expression analysis in BALB/c mice and homozygous B-hCTLA4 mice(C) by flow cytometry. Splenocytes were collected from wild type BALB/c mice (+/+) and homozygous B-hCTLA4 mice (H/H) that stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CTLA4 antibodies. Mouse CTLA4 was detectable in different T cell subtypes of wild-type BALB/c mice, while human CTLA4 was exclusively detectable in T cell subtypes of B-hCTLA4 mice(C) (H/H).
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Summary
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Protein expression analysis
Human CTLA4 was detectable in T cell subtypes of heterozygous B-hCTLA4 mice(C).
