Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hCX3CL1 mice.
The exons 2-3 of mouse Cx3cl1 gene that encode the extracellular domain were replaced by human CX3CL1 exons 2-3 in B-hCX3CL1 mice. -
mRNA expression analysis
-
Strain specific analysis of CX3CL1 gene expression in wild-type C57BL/6 mice and B-hCX3CL1 mice by RT-PCR. Mouse Cx3cl1 mRNA was detectable in brain of wild-type C57BL/6 mice (+/+). Human CX3CL1 mRNA was detectable only in homozygous B-hCX3CL1 mice (H/H) but not in wild-type mice.
-
Protein expression analysis
-
Strain specific CX3CL1 expression analysis in homozygous B-hCX3CL1 mice by ELISA. The brain homogenates was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCX3CL1 mice (H/H), and analyzed by ELISA with species-specific CX3CL1 ELISA kit. Mouse CX3CL1 was detectable in wild-type mice. Human CX3CL1 was detectable in homozygous B-hCX3CL1 mice, these two ELISA kits cross-identify human and mouse.
-
Summary
-
mRNA expression analysis:
Mouse CX3CL1 mRNA was detectable in wild-type (+/+). Human CX3CL1 mRNA was detectable in B-hCX3CL1 mice (H/H) mice but not in wild-type mice.Protein expression analysis:
Mouse CX3CL1 was detectable in wild-type mice. Human CX3CL1 was detectable in homozygous B-hCX3CL1 mice, these two ELISA kits cross-identify human and mouse.
