B-hCXCR5 mice

Basic Information

Strain Name
C57BL/6-Cxcr5tm1(CXCR5)/Bcgen
Stock Number
110970
Common Name
B-hCXCR5 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
CXCR5(C-X-C motif chemokine receptor 5), BLR1, CD185, MDR15
Species
C57BL/6
Appearance
Black
Genotypes
Heterozygous

Description

C-X-C chemokine receptor type 5 (CXC-R5) also known as CD185 (cluster of differentiation 185) or Burkitt lymphoma receptor 1 (BLR1) is a G protein-coupled seven transmembrane receptor for chemokine CXCL13 (also known as BLC) and belongs to the CXC chemokine receptor family. CXCR5 is specifically expressed in Burkitt’s lymphoma and lymphatic tissues, such as follicles in lymph nodes as well as in spleen. The gene plays an essential role in B cell migration. Through CXCL13 secretions B cells are able to locate the lymph node. Additionally, some recent studies have suggested that CXCL13, through CXCR5, is capable of recruiting hematopoietic precursor cells (CD3− CD4+) which would cause the development of lymph nodes and Peyer’s Patches. Other studies highlight the role of CXCR5 in T cells, as they are unable to access B cell follicles without CXCR5 expression. This is a key step in the production of high affinity antibodies as B cells and T cells need to interact in order to activate the Ig class switch. Recently, it was shown that CXCR5 overexpression in breast cancer patients highly correlates with lymph node metastases, and elevated CXCR5 expression may contribute to abnormal cell survival and migration in breast tumors that lack functional p53 protein. While chemokines and chemokine receptors have been thought to be involved in cancer development and maintenance, recently CXCR5 has come under investigation for its role in metastatic progression of prostate cancer. Besides, CXCR5 is also involved in the autoimmune diseases including multiple sclerosis.

Targeting strategy

Targeting strategy

Gene targeting strategy for B-hCXCR5 mice. The exons 1~2 of mouse Cxcr5 gene that encode the full-length protein were replaced by human CXCR5 exons 1~2 in B-hCXCR5 mice.

Details

Protein expression analysis

Strain specific CXCR5 expression analysis in heterozygous B-hCXCR5 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCXCR5 (H/+) mice, and analyzed by flow cytometry with species-specific CD24 antibody. Mouse CXCR5 was detectable in WT mice and heterozygous B-hCXCR5. Human CXCR5 was exclusively detectable in heterozygous B-hCXCR5 but not WT mice.

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