Basic Information
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mRNA expression analysis
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Species-specific FAP gene expression analysis in wild-type and B-hFAP mice by RT-PCR. Murine colon cancer MC38 (5×105) and melanoma B16-F10 (2×105) cells were subcutaneously implanted into wild-type C57BL/6 mice, homozygous B-hFAP mice and homozygous B-Fap KO mice. An MC38 cell line over-expressing human FAP (B-CAG-hFAP MC38) was used as a positive control. Murine Fap mRNA was detected in kidney tissue isolated from wild-type C57BL/6 mice, while human FAP mRNA was exclusively detected in B-hFAP mice and B-CAG-hFAP MC38 cells. Murine Fap and human FAP mRNA were not detected in homozygous B-Fap KO mice.
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Protein expression analysis
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Species-specific FAP protein expression analysis in wild-type, homozygous B-hFAP and B-Fap KO mice by flow cytometry. Murine melanoma B16-F10 (2×105) cells were subcutaneously implanted into wild-type C57BL/6 mice, homozygous B-hFAP mice and homozygous B-Fap KO mice. Tumor tissue was collected from the three mice, and analyzed by flow cytometry using species-specific anti-hFAP antibodies. Human FAP protein was exclusively detected in CD45- tumor cells from homozygous B-hFAP mice but not in wild-type C57BL/6 mice or B-Fap KO mice.
