Basic Information
Description
The neonatal Fc receptor (The neonatal Fc receptor (FcRn), which is similar in structure to the MHC class I molecule and also associates with beta-2-macroglobulin, it is found in the placenta to help facilitate transport of mother’s IgG to the growing fetus in humans. FcRn extends the half-life of IgG and serum albumin by reducing lysosomal degradation in endothelial cells and bone-marrow derived cells. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine, TNF-α, and down-regulated by IFN-γ.
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Targeting strategy
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Gene targeting strategy for B-hFcRn mice. The human full-length FCGRT cDNA sequence was inserted in the Fcgrt exon2 of wild-type mice
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Details
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Pharmacokinetic experiments of hIgG based on B-hFcRn mice
There was no significant difference in clearance of hIgG between B-hFcRn mice and C57BL/6 mice. Serum hIgG is still detectable with ELISA assay 2 weeks after tail intravenous injection (IV) of human IgG (10 mg/kg ) in both B-hFcRn mice and C57BL/6 mice .
Pharmacokinetic experiments of Ipilimumab based on B-hFcRn mice
There was no significant difference in clearance of Ipilimumab (IgG1) between B-hFcRn mice and C57BL/6 mice at 10 mg/kg（n=5）. Serum Ipilimumab is still detectable with ELISA assay 3 weeks after tail intravenous injection (IV) of Ipilimumab in both B-hFcRn mice and C57BL/6 mice .
Pharmacokinetic experiments of hIgG based on B-hFcRn mice
