Basic Information
Description
The neonatal Fc receptor (The neonatal Fc receptor (FcRn), which is similar in structure to the MHC class I molecule and also associates with beta-2-macroglobulin, it is found in the placenta to help facilitate transport of mother’s IgG to the growing fetus in humans. FcRn extends the half-life of IgG and serum albumin by reducing lysosomal degradation in endothelial cells and bone-marrow derived cells. Neonatal Fc receptor expression is up-regulated by the proinflammatory cytokine, TNF-α, and down-regulated by IFN-γ.
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Targeting strategy
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Gene targeting strategy for B-hFcRn mice. The human full-length FCGRT cDNA sequence was inserted in the Fcgrt exon2 of wild-type mice
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Details
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Pharmacokinetic experiments of hIgG based on B-hFcRn mice
There was no significant difference in clearance of hIgG between B-hFcRn mice and C57BL/6 mice. Serum hIgG is still detectable with ELISA assay 2 weeks after tail intravenous injection (IV) of human IgG (10 mg/kg ) in both B-hFcRn mice and C57BL/6 mice .
Pharmacokinetic experiments of Ipilimumab based on B-hFcRn mice
There was no significant difference in clearance of Ipilimumab (IgG1) between B-hFcRn mice and C57BL/6 mice at 10 mg/kg（n=5）. Serum Ipilimumab is still detectable with ELISA assay 3 weeks after tail intravenous injection (IV) of Ipilimumab in both B-hFcRn mice and C57BL/6 mice .
Pharmacokinetic experiments of hIgG based on B-hFcRn mice
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Publications using B-hFcRn mice
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- Wu F, Qiu Y, Xu Y. A single-valent long-acting human CD47 antagonist enhances antibody directed phagocytic activities. Cancer Immunol Immunother. 2020 Dec;69(12):2561-2569. doi: 10.1007/s00262-020-02640-6. Epub 2020 Jun 24. PMID: 32583154.
- Liu P, Pan Z, Gu C, Cao X, Liu X, Zhang J, Xiao Z, Wang X, Guo H, Ju D, Deng SJ. An Omalizumab Biobetter Antibody With Improved Stability and Efficacy for the Treatment of Allergic Diseases. Front Immunol. 2020 Nov 27;11:596908. doi: 10.3389/fimmu.2020.596908. PMID: 33329588; PMCID: PMC7728613.
