Basic Information

Strain name
C57BL/6-Ighetm1(IGHE)Fcer1atm1(FCER1A)/Bcgen
Common name
B-hIgE/hFCER1A mice
Background
C57BL/6N
Catalog number
111891
Aliases
IGHE, IgE; FCER1A, FCE1A, FcERI, Fc fragment of IgE receptor Ia
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hIgE/hFCER1A mice. Exons 1-6 of mouse Ige gene that encode the full-length protein were replaced by human IGHE exons 1-6 in B-hIgE/hFCER1A mice. Exons 1-5 of mouse Fcer1a gene that encode the extracellular domain were replaced by human FCER1A exons 3-7 in B-hIgE/hFCER1A mice.

Protein expression analysis

Species-specific IgE protein expression analysis in wild-type and humanized B-hIgE/hFCER1A mice. Following LPS stimulation in vivo, bone marrow-derived basophilic granulocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hIgE/hFCER1A (H/H) mice and analyzed by flow cytometry using species-specific anti-IgE antibodies. Murine IgE protein was detected in wild-type mice, while human IgE protein was detected in B-hIgE/hFCER1A mice.

Speciesspecific FCER1A protein expression analysis in wild-type and humanized B-hIgE/hFCER1A mice. Bone marrow-derived basophilic granulocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hIgE/hFCER1A (H/H) mice and analyzed by flow cytometry using species-specific anti-FCER1A antibodies. Murine FCER1A protein was detected in wild-type mice, while human FCER1A protein was detected in B-hIgE/hFCER1A mice.